Research groups
Colleges
Websites
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What controls enzymes which damage cartilage?
Theme lead, OA Centre
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The Journal of Biological Chemistry
Editorial Board Member
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Committee Member
British Society for Matrix Biology
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The Journal of Biological Chemistry
Editorial Board Member
Linda Troeberg
PhD
Honorary Departmental Research Associate.
Targeting the LRP1/HS axis in osteoarthritis and inflammation
My research programme is focused on understanding the molecular pathogenesis of chronic degenerative diseases such as osteoarthritis, with the goal of developing novel therapies to treat these conditions. We focus specifically on defining how the availability of anabolic growth factors and metalloprotease inhibitors is regulated by their trafficking between pericellular heparan sulfate proteoglycans and the cell surface endocytic receptor LRP1.
Defining molecular pathways that drive cartilage loss in osteoarthritis
Osteoarthritis is characterised by an increase in the activity of metalloproteases that degrade cartilage extracellular matrix components, and so impair the structural integrity of the tissue. This is caused by increased expression of these metalloproteases, as well as by a decrease in levels of their endogenous inhibitor, tissue inhibitor of metalloproteinases 3 (TIMP-3). Our group established that levels of TIMP-3 in cartilage are regulated by the equilibrium between its binding to heparan sulfate (HS) glycans in the extracellular matrix and its cellular endocytosis by the scavenger receptor, LDL receptor-related protein 1 (LRP1). Current projects focus on defining how HS sulfation patterns change in OA, and designing small molecule inhibitors to target TIMP-3 loss as a therapy for osteoarthritis.
LRP1/HS axis also regulates macrophage behaviour
We found that LRP1 also dynamically regulates TIMP-3 levels in macrophages, where TIMP-3 is the primary inhibitor of TNF release by the metalloproteinase ADAM17. This enables LRP1 to control termination of TNF release during the inflammatory response. As in chondrocytes, macrophage HS protects TIMP-3 and other ligands from uptake by LRP1. We are examining changes in expression of HS biosynthesis enzymes and core proteins upon macrophage phenotype and activation, and looking at how this alters retention and biological activity of HS-binding cytokines, chemokines and growth factors during inflammation.
Key publications
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Journal article
Yamamoto K. et al, (2015), Matrix Biol, 44-46, 255 - 263
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Journal article
Doherty CM. et al, (2016), J Biol Chem, 291, 22160 - 22172
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Journal article
Troeberg L. et al, (2014), Chem Biol, 21, 1300 - 1309
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Journal article
Scilabra SD. et al, (2013), J Biol Chem, 288, 332 - 342
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Journal article
Troeberg L. et al, (2012), Biochem J, 443, 307 - 315
Recent publications
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Journal article
Mohammed RN. et al, (2019), Sci Rep, 9
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Journal article
Chanalaris A. et al, (2019), Am J Pathol, 189, 632 - 647
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Journal article
Schubert K. et al, (2019), J Immunol, 202, 1501 - 1509
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Journal article
Swart M. and Troeberg L., (2019), J Histochem Cytochem, 67, 9 - 27
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Journal article
Collins LE. and Troeberg L., (2019), J Leukoc Biol, 105, 81 - 92