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Cell DIVE Multiplex Imaging

Multiplexed image of human FFPE IBD Colon tissue imaged at 20x with GE Cell DIVE. Immunofluorescence image (Left) displays 21 protein markers overlayed with DAPI alongside respective virtual H&E image (Right).
Multiplexed image of human FFPE IBD Colon tissue imaged at 20x with GE Cell DIVE. Immunofluorescence image (Left) displays 21 protein markers overlayed with DAPI alongside respective virtual H&E image (Right).

IN Cell Analyzer 2500HS and Cell DIVE multiplexed imaging

The IN Cell 2500HS is a widefield imaging system optimised for rapid image acquisition and high-throughput capabilities. The IN Cell Analyzer can perform fluorescence and transmitted light imaging with 2D, 2.5D or 3D modes in addition to integrated deconvolution. Furthermore, the system allows for multiplexed tissue imaging using Cell DIVE for the visualisation, identification and quantification of multiple markers on a tissue sample. This is achieved by iteratively staining, imaging and inactivating a single tissue slide. Spatial biomarker and single cell analysis can then be performed, allowing the ability for cell phenotyping in a preserved spatial context to uncover previously hidden cell-cell interactions in disease processes.

Microscope Objectives
• Nikon 2X/NA 0.1
• Nikon 10X/NA 0.45
• Nikon 20X/NA 0.75
• Nikon 40x/NA 0.95

Supported dyes
• Blue (DAPI, Hoechst, CF™405M)
• Green (GFP, Cy™2, Alexa Fluor™ 488, ATTO-488, CellTracker™ Green, Calcein AM)
• Orange (Cy3, Alexa Fluor 546, TRITC, propidium iodide, CellTracker Orange, CellTracker CM-DIL, mOrange)
• Red (mCherry, mKate2, AlexaFluor 568, rhodamine-phalloidin, CellTracker Red)
• Far Red (Cy5, AlexaFluor 647, To-Pro™-3, SiR)

Detectors
• sCMOS camera
• 2040 × 2040 imaging array
• 6.5 × 6.5 μm pixels
• 16-bit dynamic range
• 272.3 MHz readout speed
• 0.9 (median) / 1.4 (rms) readout noise

Software
• IN Cell Analyzer 2500
• IN Cell Developer Toolbox
• QuPath
• ImageApp
• Layers

For more information on booking, training and pricing, please email the DPOC Facility.

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