Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We report a novel, compact and automated multidimensional spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Förster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

Original publication




Journal article


J Biophotonics

Publication Date





494 - 505


Cartilage, Cell Membrane, Equipment Design, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Humans, In Vitro Techniques, Lipid Bilayers, Microscopy, Fluorescence, Multiphoton, Optical Fibers, Photochemical Processes, Protein Interaction Mapping, Pyridinium Compounds, Spectrometry, Fluorescence