Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Interleukin 10 is a potent anti-inflammatory and immunomodulatory cytokine. Little is known regarding its induction in monocytes/macrophages, however LPS, a reproducible trigger of IL-10, is augmented by direct contact with T cells. In this context, the role of CD40-ligation is investigated. In the rheumatoid synovium, IL-10 is produced by tissue macrophages. Monocytes primed with M-CSF, a cytokine present in rheumatoid joints, produced IL-1beta, TNF-alpha and IL-10 upon CD40-ligation at an IL-1: TNF-alpha: IL-10 ratio of 10:0.5:1. IFN-gamma-primed monocytes, however, predominantly produced TNF-alpha and IL-1beta. Both differentiated monocytes display an endogenous IL-10 activity regulatable by CD40 stimulation. Additionally, these monocytes display differential control by exogenous and endogenous IL-1 and TNF-alpha. M-CSF-primed monocyte IL-10 production was dependent on endogenous TNF-alpha and, to a lesser extent, IL-1, whereas IFN-gamma-primed monocytes were partially dependent on endogenous IL-1. The addition of exogenous IL-1 augments CD40 induced IL-10 production by IFN-gamma-primed monocytes. These data indicate that CD40 ligation regulates cell contact mediated macrophage IL-10 and that the route of differentiation determines the cytokine profile.

Original publication

DOI

10.1006/cyto.2000.0750

Type

Journal article

Journal

Cytokine

Publication Date

10/2000

Volume

12

Pages

1496 - 1505

Keywords

Antigens, CD40, Cell Differentiation, Cell Membrane, Coculture Techniques, Cytokines, Enzyme-Linked Immunosorbent Assay, Humans, Interferon-gamma, Interleukin-1, Interleukin-10, Leukocytes, Mononuclear, Lipopolysaccharides, Macrophage Colony-Stimulating Factor, Macrophages, Monocytes, Phenotype, Signal Transduction, Time Factors, Transfection, Tumor Necrosis Factor-alpha