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FACSCanto II

FACSCanto II

Overview

The FACSCanto II is a digital three-laser multi-colour analysis system (Blue 488 nm, Red 633 nm and Violet 405 nmwith the capacity of detecting up to 8 colours in addition to FSC and SSC.  

Detailed information

The instrument has the optional High Throughput Sampler (HTS) that facilitates automated, rapid sample acquisition from a 96 well plate with minimal carry over.

The instrument allows a minimum sample flow rate of 10 ul/min to a maximum flow rate of 120 ul/min.

The instrument contains an integrated fluidics cart and compressor, a 20 L FACSFLOW container, 10 L waste tank and cleaning solutions for automated startup, shutdown and cleaning cycles.  

 

FACSCanto II Configuration
LASER COLOUR FILTER MIRROR
Blue 488 nm FITC, Alexa 488 530/30 502 LP
  PE 585/42 556LP
 

PerCP, PerCP-CY5.5

PE-Cy5, PE-Cy5.5

670LP

655LP

  PE-Cy7 780/60 735LP
Red 633 nm APC, Alexa 647 660/20  
  APC-Cy7, APC-H7 780/60 735LP
Violet 405 nm Pacific Blue, BV421 450/50  
  AmCyan, BV510 510/50 502LP
       

PE-Cy5 and APC may be used together for molecules that are not mutually expressed by cells.