Multi-photon microscopy
ZEISS LSM 880 NLO confocal Multiphoton microscope
GENERAL SPECIFICATIONS
- ZEISS LSM 880 NLO confocal Multiphoton system on Axio Examiner Z1 upright fixed stage microscope
- Heated chamber for organ explant imaging and temperature control
- 2 Mai-Tai laser for simultaneous 2-photon excitation at 2 different wavelengths
- Scan speed up to 19 × 2 speed levels, up to 13 images/sec. For 512 × 512 pixels (max. 430 images/sec), up to 6875 lines per second
- xy, xz, xyz, xyzt, lambda, line scan and spot scan modes
MICROSCOPE OBJECTIVES
- W Plan-APO 20X1.0 DIC VIS-IR D=0.17
- W Plan-APO 20X1.0 DIC VIS-IR D=0
ILLUMINATION
- Lamps for bright field, DIC and epifluorescent imaging
- Mai-Tai tuneable laser 690-1040 nm, pulse width <70 fs
- Mai-Tai tuneable BB laser 710–990 nm, pulse width <80 fs
- Argon laser (458, 488, 514), 543 and 633 lasers
DETECTORS
- 2 x Nosepiece GaAsP : BP395-455 + BP465-485 OR BP500-550 + SP485
- 2 x GaAsP (BiG): BP529.5-554.5 + LP555 OR BP500-550 + BP590-625
- 2 x PMT NDD detectors: BP595-676 + BP490-530 OR BP645-690 + SP485
- 34 descanned spectral channels
SOFTWARE
- Zeiss Zen software
- Mosaic and stitching imaging
- Time-lapse experiments
ADD-ONS
- The imaging system is supplemented with dissection equipment, laminar flow hood, and anaesthesia equipment