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Artificial lipid bilayers are powerful tools that can be used to model the interactions between platelets and membrane-bound ligands. To mimic the interaction of platelets with membrane-bound ligands, biotinylated lipids can be used to couple monobiotinylated recombinant ligands to the upper leaflet of an artificial lipid bilayer using streptavidin to bridge the two. Artificial lipid bilayers are generated by preparing liposomes, treating glass coverslips to make them hydrophilic and by assembling the bilayer in a specialized flow chamber. Finally platelets can be added to the flow chamber and the localization of fluorescently labeled molecules followed using microscopy.

Original publication

DOI

10.1007/978-1-4939-8585-2_8

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2018

Volume

1812

Pages

127 - 137

Keywords

Artificial lipid bilayer, CLEC-2, Podoplanin, Receptor–ligand interactions