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Studies of hematopoietic stem cell (HSC)-derived dendritic cells (DCs) are often limited by the rarity of HSC. To facilitate the study of DCs, we have generated a novel cell line (CR1) by retroviral Notch(IC) gene transfer into Sca1(+)ckit(+)lin- HSC. CR1 cells proliferated in vitro in the presence of recombinant interleukin-3. They maintained an immature progenitor cell phenotype and an intact karyotype. In the presence of granulocyte-macrophage colony-stimulating factor or Flt3L, CR1 cells differentiated into myeloid and plasmacytoid DCs, respectively. Functionally, CR1 cells were comparable to primary bone-marrow-derived DCs with respect to Toll-like-receptor-mediated maturation, cytokine release and capacity to induce effective antitumor immunity. CR1 cells thus provide an elegant new cellular tool to study DC development, function and preclinical DC-based immunotherapies.

Original publication




Journal article



Publication Date





870 - 876


Cell Differentiation, Cell Line, Cell Proliferation, Dendritic Cells, Granulocyte-Macrophage Colony-Stimulating Factor, Hematopoietic Stem Cells, Humans, Immunophenotyping, Immunotherapy, In Vitro Techniques, Interleukin-3, Karyotyping, Leukemia, Membrane Proteins, Recombinant Proteins, Retroviridae, Transfection