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In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.

Original publication

DOI

10.1038/ni1139

Type

Journal article

Journal

Nat Immunol

Publication Date

12/2004

Volume

5

Pages

1243 - 1250

Keywords

Animals, Antigens, CD11c, Cell Communication, Cell Differentiation, Cell Movement, Dendritic Cells, Lymph Nodes, Mice, Mice, Transgenic, Microscopy, Confocal, Microscopy, Fluorescence, Recombinant Proteins, T-Lymphocytes