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A human helper T cell line (F14m) activated by the matrix protein purified from A/X31 influenza virus has been developed. After activation by antigen for 7 days, and reculture with matrix protein and irradiated autologous feeder cells, the cells obtained from an in vivo influenza virus-immunized donor have been growing in the presence of interleukin 2 for more than 7 months. The cells all belong to the helper-inducer T cell subset and most of them express surface membrane HLA-DR antigens. A small number (approximately 10(3)) of F14m T cells provided optimal help for 1 X 10(5) autologous T-depleted lymphocytes for production of anti-A/X31 but not anti-B/HK antibodies. The F14m T cells produce soluble factors (S14m) able to help B cells to secrete anti-A/X31 antibodies. F14m and S14m were shown to help antibody production to hemagglutinin when cultured with B cells and the whole virus. The specificity of the T cell line for type-A matrix protein was confirmed by the ability of S14m to provide help for anti-A/JAP (A/H2/N2) but not for anti-B/HK antibody production. These data provide evidence for matrix protein-specific T helper cells and factors able to provide help for antibody production against hemagglutinin, a distinct protein of the same virus.

Original publication

DOI

10.1002/eji.1830121009

Type

Journal article

Journal

Eur J Immunol

Publication Date

10/1982

Volume

12

Pages

844 - 849

Keywords

Antibodies, Monoclonal, B-Lymphocytes, Cell Line, Epitopes, Hemagglutinins, Humans, Influenza A virus, Interleukin-2, T-Lymphocytes, T-Lymphocytes, Helper-Inducer