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Cell-autonomous innate immune responses against bacteria attempting to colonize the cytosol of mammalian cells are incompletely understood. Polyubiquitylated proteins can accumulate on the surface of such bacteria, and bacterial growth is restricted by Tank-binding kinase (TBK1). Here we show that NDP52, not previously known to contribute to innate immunity, recognizes ubiquitin-coated Salmonella enterica in human cells and, by binding the adaptor proteins Nap1 and Sintbad, recruits TBK1. Knockdown of NDP52 and TBK1 facilitated bacterial proliferation and increased the number of cells containing ubiquitin-coated salmonella. NDP52 also recruited LC3, an autophagosomal marker, and knockdown of NDP52 impaired autophagy of salmonella. We conclude that human cells utilize the ubiquitin system and NDP52 to activate autophagy against bacteria attempting to colonize their cytosol.

Original publication

DOI

10.1038/ni.1800

Type

Journal article

Journal

Nat Immunol

Publication Date

11/2009

Volume

10

Pages

1215 - 1221

Keywords

Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Autophagy, HeLa Cells, Humans, Microtubule-Associated Proteins, Molecular Sequence Data, Nuclear Proteins, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, RNA Interference, Salmonella Infections, Salmonella enterica, Ubiquitin, Ubiquitination