Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Aggrecanases are considered to play a key role in the destruction of articular cartilage during the progression of arthritis. Here we report that the N-terminal inhibitory domain of tissue inhibitor of metalloproteinases 3 (N-TIMP-3), but not TIMP-1 or TIMP-2, inhibits glycosaminoglycan release from bovine nasal and porcine articular cartilage explants stimulated with interleukin-1alpha or retinoic acid in a dose-dependent manner. This inhibition is due to the blocking of aggrecanase activity induced by the catabolic factors. Little apoptosis of primary porcine chondrocytes is observed at an effective concentration of N-TIMP-3. These results suggest that TIMP-3 may be a candidate agent for use against cartilage degradation.

Original publication




Journal article



Publication Date





431 - 436


Aggrecans, Animals, Apoptosis, Cartilage, Articular, Cattle, Cell Line, Chondrocytes, Culture Techniques, Endopeptidases, Extracellular Matrix Proteins, Glycosaminoglycans, Humans, Interleukin-1, Lectins, C-Type, Protease Inhibitors, Protein Structure, Tertiary, Proteoglycans, Recombinant Proteins, Swine, Tissue Inhibitor of Metalloproteinase-3, Tretinoin