LSR II
Overview
The LSR II is a digital three-laser multi-colour analysis system (Blue 488 nm, Red 633 nm and Violet 405 nm) with the capacity of detecting up to 14 colours in addition to FSC and SSC.
Detailed information
| LASER | COLOUR | FILTER | MIRROR |
|---|---|---|---|
| Blue 488 nm | FITC, Alexa 488 | 530/30 | 505 LP |
| PE | 575/25 | 550LP | |
| PerCP, PerCP-CY5.5 | 695/40 | 685LP | |
| PE-Cy5, PE-Cy5.5 | 660/20 | 655LP | |
| PE-Texas Red | 610/20 | 600LP | |
| PE-Cy7 | 780/60 | 755LP | |
| Red 633 nm | APC, Alexa 647 | 660/20 | |
| APC-Cy7, APC-H7 | 780/60 | 755LP | |
| APC-Alexa 700 | 730/45 | 710LP | |
| APC-680 | 635/35 | 675LP | |
| Violet 405 nm | Pacific Blue, BV421 | 450/50 | |
| AmCyan, BV510 | 525/50 | 505LP | |
| BV605 | 610/20 | 595LP | |
| BV650 | 670/30 | 630LP | |
| BV711 | 710/50 | 690LP | |
| BV785 | 780/50 | 750LP |
- Please note – PE-Cy5, PE-Cy5.5, perCP and PerCP-Cy5.5 cannot be used together.
- PE-Cy5 and APC may be used together for molecules that are not mutually expressed by cells.
- Alexa -700 and Alexa- 680 cannot be used together.
LSR II has the capability for adjusting the flow rate for various applications. The three preset flow rates are: LO = 12 µl/min, MED = 35 μl/min, HI = 60 μl/min.
LSR II is equipped with FACSFLOW supply system fluidics cart that incorporate a 20 L FACSFLOW cubitainer and a 10 L waste collection container. The Fluidics sensors monitor the level of sheath fluid or waste and warn when sheath fluid is low or empty, or when the waste container is full.
Our team
-
Jonathan Webber
Flow Cytometry Facility Manager
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