A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells
Abu-Shah E., Demetriou P., Mayya V., Balint S., Kutuzov M., Dushek O., Dustin M.
Abstract Research in the field of human immunology is restricted by the lack of a system that reconstitutes the in-situ activation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation.