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Intercellular adhesion molecule-3 (ICAM-3, CD50), a member of the immunoglobulin gene superfamily, is a major ligand for the lymphocyte function-associated antigen 1 (LFA-1, CD18/CD11a) in the resting immune system and plays a role as a signaling and costimulatory molecule on T lymphocytes. In this study we have generated a large panel of anti-ICAM-3 monoclonal antibodies (mAb) and show that the biological effects of these antibodies are critically dependent on the epitope recognized. By using an adhesion assay employing COS cells expressing LFA-1 binding to recombinant chimeric ICAM-3-Fc proteins (which overcomes the confounding effects of interleukocyte LFA-1/ICAM binding events), we have been able to examine the effects of these antibodies in blocking LFA-1/ICAM-3 adhesion. Our data suggests that only a small minority of ICAM-3 mAb, recognizing a distinct epitope, are able to mimic the effects of LFA-1 binding to ICAM-3. Moreover these antibodies are functionally distinct as defined by their costimulatory activity and ability to elicit interleukin-2 production and cell proliferation in T lymphocytes.

Original publication

DOI

10.1002/eji.1830250223

Type

Journal article

Journal

Eur J Immunol

Publication Date

02/1995

Volume

25

Pages

459 - 465

Keywords

Animals, Antibodies, Monoclonal, Antigens, CD, Antigens, Differentiation, Cell Adhesion Molecules, Cell Aggregation, Cell Line, Epitope Mapping, Humans, Lymphocyte Activation, Lymphocyte Function-Associated Antigen-1, Mice, Mice, Inbred BALB C