Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Studies of hematopoietic stem cell (HSC)-derived dendritic cells (DCs) are often limited by the rarity of HSC. To facilitate the study of DCs, we have generated a novel cell line (CR1) by retroviral Notch(IC) gene transfer into Sca1(+)ckit(+)lin- HSC. CR1 cells proliferated in vitro in the presence of recombinant interleukin-3. They maintained an immature progenitor cell phenotype and an intact karyotype. In the presence of granulocyte-macrophage colony-stimulating factor or Flt3L, CR1 cells differentiated into myeloid and plasmacytoid DCs, respectively. Functionally, CR1 cells were comparable to primary bone-marrow-derived DCs with respect to Toll-like-receptor-mediated maturation, cytokine release and capacity to induce effective antitumor immunity. CR1 cells thus provide an elegant new cellular tool to study DC development, function and preclinical DC-based immunotherapies.

Original publication

DOI

10.1038/sj.leu.2404157

Type

Journal article

Journal

Leukemia

Publication Date

05/2006

Volume

20

Pages

870 - 876

Keywords

Cell Differentiation, Cell Line, Cell Proliferation, Dendritic Cells, Granulocyte-Macrophage Colony-Stimulating Factor, Hematopoietic Stem Cells, Humans, Immunophenotyping, Immunotherapy, In Vitro Techniques, Interleukin-3, Karyotyping, Leukemia, Membrane Proteins, Recombinant Proteins, Retroviridae, Transfection