Live-cell super-resolution eTIRF-SIM system

GENERAL SPECIFICATIONS:

eTIRF-SIM (extended Total Internal Reflection Fluorescence  Structured Illumination) is a state of the art super-resolution microscope. It takes advantage of combining two imaging modalities TIRF and SIM in the same system, which allows achieving up to 90 nm axial and 150 nm lateral resolutions at very high acquisition rates – up to 100 ms per colour per frame. Moreover, TIRF illumination helps to reduce phototoxicity and photodamage as the sample is only illuminated at the basal plain leaving majority of it intact. This makes TIRF-SIM an ideal candidate for high resolution live cell imaging.

MICROSCOPE OBJECTIVES:

• Olympus UApoN100× NA 1.49

ILLUMINATION:

• Lasers: 488 nm, 560 nm, and 650 nm

DETECTORS:

• Camera-based

LIVE-CELL ENVIORMENT CONTROL:

• OKO lab incubator with temperature and CO2 control

SOFTWARE:

• Custom-built

ACKNOWLEDGMENT:

We acknowledge the Wellcome Trust (212343/Z/18/Z), and EPSRC (EP/S004459/1) grants. The TIRF-SIM microscope was built in collaboration with and with funds from Micron, an Oxford-wide advanced microscopy technology consortium supported by Wellcome Strategic Awards (091911 and 107457).

Contact:

Dr Marco Fritzsche marco.fritzsche@rdm.ox.ac.uk

Dr Kseniya Korobchevskaya kseniya.korobchevskaya@kennedy.ox.ac.uk