Flow cytometry
Flow cytometry is an essential technique for many cell biology or immunology applications. The KIR flow cytometry facility is managed by Jonathan Webber under academic supervision of Prof Lynn Dustin, and supports around 70 active users.
Equipment
The KIR flow facility operates 2 BD analysers (LSRII, Fortessa X20), 1 Cytek spectral analyser (Aurora), and 1 BD cell sorter (Aria III).
The KIR flow facility also provides a Meso Scale Quickplex SQ120 imager, for detection of up to 10 soluble analytes simultaneously.
- LSR II (R/B/V), Fortessa X20 (R/B/V/YG) are 14-colour maximum panel size. The YG laser on the Fortessa offers an advantage with dsRed/mCherry/tdTomato reporters. The Fortessa has a plate loader.
- Aurora (R/B/V/YG/UV) is a spectral cytometer capable of a 40-colour panel size, with a very effective plate loader for automation. A major part of the KIR scientific remit is immune/stromal crosstalk. This is facilitated by autofluorescence extraction available on the Aurora, whilst the large panel size enables multiplexing of several smaller panels.
- Aria III (R/B/V/YG), configuration equivalent to the Fortessa. Cell sorter is enclosed in a BSC for safe sorting of unscreened blood. Can sort 4 populations simultaneously or into single wells of a 96-well plate. Maximum throughput around 50 million cells/hr for PBMC/splenocyte or similar.
Support
The flow facility provides Flowjo portal licenses for data analysis. The facility manager can assist with experiment design, troubleshooting and analysis. If unsure, please email before reagent purchase!
Cell sorting is operator-run in the first instance. The flow manager will discuss your experimental requirements (viability, downstream processing, input number and expected yields, duration, biosafety) prior to scheduling, in order to maximize likelihood of good experimental outcome.
Useful links
We have curated a comprehensive list of useful links of all things flow cytometry.
Publications
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IRAK3 is upregulated in rheumatoid arthritis synovium and delays the onset of experimental arthritis
Journal article
Borghese F. et al, (2025), Frontiers in Immunology, 16
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A Single-Domain VNAR Nanobody Binds with High-Affinity and Selectivity to the Heparin Pentasaccharide Fondaparinux
Journal article
Gschwandtner M. et al, (2025), International Journal of Molecular Sciences, 26, 4045 - 4045
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Permissive central tolerance plus defective peripheral checkpoints license pathogenic memory B cells in CASPR2-antibody encephalitis.
Journal article
Sun B. et al, (2025), Sci Adv, 11
-
Systemic inflammation impairs myelopoiesis and interferon type I responses in humans.
Journal article
Keramati F. et al, (2025), Nat Immunol
Latest publications
-
IRAK3 is upregulated in rheumatoid arthritis synovium and delays the onset of experimental arthritis
Journal article
Borghese F. et al, (2025), Frontiers in Immunology, 16
-
A Single-Domain VNAR Nanobody Binds with High-Affinity and Selectivity to the Heparin Pentasaccharide Fondaparinux
Journal article
Gschwandtner M. et al, (2025), International Journal of Molecular Sciences, 26, 4045 - 4045
-
Permissive central tolerance plus defective peripheral checkpoints license pathogenic memory B cells in CASPR2-antibody encephalitis.
Journal article
Sun B. et al, (2025), Sci Adv, 11
-
Systemic inflammation impairs myelopoiesis and interferon type I responses in humans.
Journal article
Keramati F. et al, (2025), Nat Immunol
Booking and access
Internal booking is available via BookIt once training has been completed. Please contact the flow manager to arrange training and access. External access (including sorting) is possible via contacting the flow manager, though internal usage is prioritised.