Olympus TIRF

Total Internal Reflection Fluorescence (TIRF)

Olympus TIRF Microscope

Ideal for dynamic processes within the plane of the coverslip for example immunological synapses and bilayer interactions. Fluorophore is only excited within ~100 nm of the focal plane (evanescent field) giving excellent 2D sectioning.

GENERAL SPECIFICATIONS

• Olympus TIRF IX83 Microscope based on IX83 motorized inverted microscope
• Compensation focusing system
• CellVivo incubator system for live-cell imaging and temperature control
• 4 diode lasers
• Freely selectable ROI for imaging, bleaching and photoactivation
• Imaging speed of up to 4-10 ms per frame (512 x 512 pixels)
• Camera splitter for simultaneous imaging in two channels
• DIC optics
• Individual control over depth penetration of evanescent laser illumination 

MICROSCOPE OBJECTIVES

• UAPON 20x /NA 0.70
• UCPLFLN 20x/NA 0.70
• UAPON 150x/NA 1.45 Oil 

ILLUMINATION

• Lamps for bright field, DIC and epifluorescent imaging
• Violet Diode Laser 405 nm
• Blue Diode Laser 488 nm
• Green Diode Laser 561 nm
• Red Diode Laser 640 nm

DETECTORS

• Evolve EMCCD 512x512 camera supplemented with Cairn camera splitter (IX3 RSD) for simultaneous imaging in two channels
• Hamamatsu Flash 2.8 camera 

ENVIRONMENT CONTROL

• Dark incubator chamber with temperature, CO2 control and lighting from CellVivo 

SOFTWARE

• Olympus cellSens Dimension