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Multi-photon microscopy

Olympus FV1200 Multi-photon laser scanning microscopy

Multi-photon

GENERAL SPECIFICATIONS

  • Olympus FV1200 Multi-Photon Laser Scanning Microscope based on IX83 motorized inverted microscope
  • Compensation focusing system
  • CellVivo incubator system for live-cell imaging and temperature control
  • Mai-Tai laser for 2-photon excitation
  • Freely selectable ROI for laser scanning
  • Scan speeds of up to 4 frames/second with 512 x 512 pixels
  • xy, xz, xyz, xyzt, lambda, line scan and spot scan modes 

MICROSCOPE OBJECTIVES

  • XL Plan N 25x/NA 1.05 WMP 

ILLUMINATION

  • Lamps for bright field, DIC and epifluorescent imaging
  • Mai-Tai tuneable laser  690-1040 nm, pulse width <70 fs 

DETECTORS

  • 2 PMT equipped with galvano grating spectral scanning systems for fluorescence emission detection with variable setting of wavelength band
  • 2 high sensitive GaAsP detection channels with filter sets 

SOFTWARE

  • Olympus FluoView
  • Positioning (up to 3000 measuring points different in X, Y and Z)
  • Mosaic and stitching imaging
  • Time-lapse experiments
  • FRET and FRAP measurements 

ADD-ONS

Multi-photon

  • The imaging system is supplemented with dissection equipment, laminar flow hood, and anaesthesia equipment 

ACKNOWLEDGEMENT

We request that publications that include data or analysis performed with the facility include the following acknowledgement- “ We acknowledge the generous support of the Kennedy Trust for Rheumatology Research for the microscopy facilities used in this research”.