GeoMx Digital Spatial Profiler

The diagram illustrates the GeoMx Digital Spatial Profiler (DSP) workflow for analysing protein or RNA targets in tissue samples.

The process is shown as a series of numbered steps:

Step 1: A slide is prepared and stained with fluorescent antibodies and GeoMx DSP probes. These probes can target either proteins or RNA molecules within the tissue.

Step 2: Regions of interest, or ROIs, are selected on the tissue using a microscope image. These regions are highlighted for further analysis.

Step 3: Ultraviolet light is used to cleave DSP barcodes from the RNA probes within the selected regions.

Step 4: The barcoded probes are dispensed into a microplate with 96 wells.

Step 5: The process of selecting regions and collecting barcodes is repeated for each region of interest across the tissue sample.

Step 6: The collected material is prepared into a library, sequenced and counted.