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GeoMx Digital Spatial Profiler (Bruker)

GEOMX Digital Spatial Profiler (Bruker)

HOW DOES IT WORK?

The GeoMx DSP allows researchers to use immunofluorescence microscopy to target Regions of Interest (ROI) within tissues for targeted or transcriptome-wide profiling.

 

GeoMxDSPhowdoesitwork.png

 

 

 

 

 

 

Source: Bruker Spatial Biology

Accessible version

APPLICATIONS
  • Whole Transcriptome gene expression
  • Targeted Spatial Proteomics (570-plex and 1,200-plex panels)

COMPATIBLE SPECIES
  • Human
  • Mouse
  • Canine

SAMPLE COMPATIBILITY
  • Fresh Frozen
  • FFPE
  • Fixed Frozen
  • Tissue microarray (TMA)

ASSAY INPUT
  • Tissues sectioned onto standard histology Superfrost Plus glass slides

INSTRUMENT RUN TIME
  • 1 - 2 days depending on number of slides per run

ANALYTICAL REGION
  • 14.1mm x 35.3mm

RESOLUTION
  • 100μm square region

DETECTABLE GENES: PROBE-BASED CHEMISTRY
  • Ready-to-use panels for GeoMx RNA assays:

GeoMx Human Whole Transcriptome Atlas (GeoMx Hu WTA) 
GeoMx Mouse Whole Transcriptome Atlas (GeoMx Mouse WTA) 
GeoMx Cancer Transcriptome Atlas (CTA)
GeoMx TCR Profiling Add-On
GeoMx 2k-plex Canine Cancer Atlas panel
GeoMx Immune Pathways panel

Please refer to Bruker’s webpage for product range. 

  • Ready-to-use panels for GeoMx Spatial Proteomics assays: 

GeoMx Discovery Proteome Atlas (1200+ pre-validated human protein targets, highest-plex commercially available antibody-based spatial proteomics assay)
GeoMx IO Proteome Atlas (panel of 570+ human protein targets. Customisable with up to 40 additional protein targets of your choice)
GeoMx Immuno-Oncology Protein Panels (panel of up to 96 human or mouse protein targets, relevant to immuno-oncology research, with nCounter readouts)
GeoMx Neuroscience Protein Panels (panel of up to 96 human or mouse protein targets, relevant to neuroscience research, with nCounter readouts)

Please refer to Bruker’s webpage for product range.

  • Customisation options available:

Spike-in up to 400 RNA targets into any NGS readout assay 
or run 2 panels of up to 200 targets each, as a standalone RNA assay
Spike in up to 40 custom antibodies to any NGS readout protein assay
or add up to 10 antibodies to any nCounter readout protein assay

  • Off-the-shelf morphology marker kits: Solid Tumour TME and Melanoma TME. 

Please speak to the SCSG Facility team for a list of recommended GeoMx morphology markers that have already been used by others.