The LSR Fortessa X-20 is a digital four-laser multi-colour analysis system (Blue 488 nm, Yellow-Green 561 nm, Red 633 nm and Violet 405 nm) with the capacity of detecting up to 14 colours in addition to FSC and SSC.
The Yellow-Green 561 nm laser does not increase fluorochrome options but increases detection sensitivity for the PE fluorochrome and eliminates PE-FITC spillover and more importantly improves resolution of red fluorescence protein such as DsRed, fruit fluorescence proteins such as mCherry or mStrawberry or vegetable fluorescence protein, such as tdTtomato.
The LSR Fortessa has the optional High Throughput Sampler (HTS) that facilitates automated, rapid sample acquisition from a 96 well plate with minimal carry over.
|Blue 488 nm||FITC||529/24||505 LP|
|Yellow-Green 561 nm||PE||586/15||570LP|
|Red 633 nm||APC||670/30||650LP|
|Violet 405 nm||Pacific Blue||450/50||410LP|
- Please note – PE-cy5, PE-Cy5.5, perCP and PerCP-Cy5.5 cannot be used together.
- PE-Cy5 and APC may be used together for molecules not mutually expressed by cells.
Fortessa X20 has the capability for adjusting the flow rate for various applications. The three preset flow rates are: LO = 12 µl/min, MED = 35 μl/min, HI = 60 μl/min.
Fortessa is equipped with FACSFLOW supply system fluidics cart that incorporate a 20 L FACSFLOW cubitainer and a 10 L waste collection container. The Fluidics sensors monitor the level of sheath fluid or waste and warn when sheath fluid is low or empty, or when the waste container is full.