Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The Dok adaptor family of proteins binding to RasGAP, consisting of Dok-1 and Dok-2, are critical regulators in cell proliferation. These molecules are partners and/or substrates of different protein tyrosine kinases considered as oncoproteins. Here, we show that Dok-1 and Dok-2 are the major tyrosine-phosphorylated proteins associated to Tec, a protein tyrosine kinase expressed in T cells. Furthermore, we evaluate the effect of Dok-1 or Dok-2 on Tec-mediated signalling pathways in T cells. Here, we provide evidence that Dok-1 and Dok-2 proteins are involved in a negative feedback regulation of Tec via a downregulation of its tyrosine phosphorylation and downstream signalling pathways including the Ras pathway. Either Dok-1 or Dok-2 therefore represents a mean of potent retrograde control for protein tyrosine kinase signalling, and then possibly of tumor development.

Original publication

DOI

10.1038/sj.onc.1207283

Type

Journal article

Journal

Oncogene

Publication Date

26/02/2004

Volume

23

Pages

1594 - 1598

Keywords

Adaptor Proteins, Signal Transducing, Animals, Carrier Proteins, Cell Line, Cell Line, Tumor, DNA-Binding Proteins, Gene Expression Regulation, Humans, Hybridomas, Jurkat Cells, Mice, Mice, Knockout, Phosphoproteins, Phosphorylation, Protein-Tyrosine Kinases, RNA-Binding Proteins, Signal Transduction, T-Lymphocytes