Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Detecting intracellular calcium signaling with fluorescent calcium indicator dyes is often coupled with microscopy techniques to follow the activation state of non-excitable cells, including lymphocytes. However, the analysis of global intracellular calcium responses both at the single-cell level and in large ensembles simultaneously has yet to be automated. Here, we present a new software package, CalQuo (Calcium Quantification), which allows the automated analysis and simultaneous monitoring of global fluorescent calcium reporter-based signaling responses in up to 1000 single cells per experiment, at temporal resolutions of sub-seconds to seconds. CalQuo quantifies the number and fraction of responding cells, the temporal dependence of calcium signaling and provides global and individual calcium-reporter fluorescence intensity profiles. We demonstrate the utility of the new method by comparing the calcium-based signaling responses of genetically manipulated human lymphocytic cell lines.

Original publication

DOI

10.1038/srep16487

Type

Journal article

Journal

Sci Rep

Publication Date

13/11/2015

Volume

5

Keywords

Aniline Compounds, Calcium, Calcium Signaling, Cell Line, Tumor, Computational Biology, Fluorescent Dyes, HEK293 Cells, Humans, Intracellular Space, Jurkat Cells, Reproducibility of Results, Single-Cell Analysis, Software, T-Lymphocytes, Time-Lapse Imaging, Xanthenes