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The integrin LFA-1 and its ligand ICAM-1 mediate B cell adhesion, but their role in membrane-bound antigen recognition is still unknown. Here, using planar lipid bilayers and cells expressing ICAM-1 fused to green fluorescence protein, we found that the engagement of B cell receptor (BCR) promotes B cell adhesion by an LFA-1-mediated mechanism. LFA-1 is recruited to form a mature B cell synapse segregating into a ring around the BCR. This distribution is maintained over a wide range of BCR/antigen affinities (10(6) M(-1) to 10(11) M(-1)). Furthermore, the LFA-1 binding to ICAM-1 reduces the level of antigen required to form the synapse and trigger a B cell. Thus, LFA-1/ICAM-1 interaction lowers the threshold for B cell activation by promoting B cell adhesion and synapse formation.

Type

Journal article

Journal

Immunity

Publication Date

05/2004

Volume

20

Pages

589 - 599

Keywords

Animals, B-Lymphocytes, Cell Adhesion, Intercellular Adhesion Molecule-1, Lipid Bilayers, Lymphocyte Activation, Lymphocyte Function-Associated Antigen-1, Mice, Mice, Transgenic, Microscopy, Confocal, Polymerase Chain Reaction, Receptors, Antigen, B-Cell