Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The immunological synapse (IS) is a junction between the T cell and antigen-presenting cell and is composed of supramolecular activation clusters (SMACs). No studies have been published on naive T cell IS dynamics. Here, we find that IS formation during antigen recognition comprises cycles of stable IS formation and autonomous naive T cell migration. The migration phase is driven by PKCtheta, which is localized to the F-actin-dependent peripheral (p)SMAC. PKCtheta(-/-) T cells formed hyperstable IS in vitro and in vivo and, like WT cells, displayed fast oscillations in the distal SMAC, but they showed reduced slow oscillations in pSMAC integrity. IS reformation is driven by the Wiscott Aldrich Syndrome protein (WASp). WASp(-/-) T cells displayed normal IS formation but were unable to reform IS after migration unless PKCtheta was inhibited. Thus, opposing effects of PKCtheta and WASp control IS stability through pSMAC symmetry breaking and reformation.

Original publication

DOI

10.1016/j.cell.2007.03.037

Type

Journal article

Journal

Cell

Publication Date

18/05/2007

Volume

129

Pages

773 - 785

Keywords

Animals, Antigen Presentation, Antigen-Presenting Cells, Cell Communication, Cell Movement, Enzyme Activation, Enzyme Inhibitors, Enzyme Repression, Intercellular Junctions, Isoenzymes, Lymphocyte Activation, Membrane Lipids, Mice, Mice, Knockout, Protein Kinase C, T-Lymphocytes, Wiskott-Aldrich Syndrome Protein