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Human peripheral blood leucocytes, cultured for 4--6 days with the appropriate concentration of protein or synthetic polypeptide antigen, yield helper cells. These cells, after a further 24 hr incubation with the appropriate antigen release helper factors, which are of two types, antigen specific and non-specific; a similar situation to that described in the mouse. These factors are assayed by their effects on the plaque-forming cell response of mouse spleen cells cultures in vitro. The potential applications of this new assay for human T cell function are discussed.

Type

Journal article

Journal

Clin Exp Immunol

Publication Date

04/1979

Volume

36

Pages

71 - 77

Keywords

Adult, Albumins, Animals, Antigens, Cells, Cultured, Epitopes, Haptens, Hemocyanin, Hemolytic Plaque Technique, Humans, Lymphocyte Cooperation, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Middle Aged, Peptides, T-Lymphocytes