Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells stimulated with CD16-binding nanoarrays. In both cases, the strength of response decreased with increasing spacing, falling to background levels by 69 nm in the T cell/anti-CD3 system and 104 nm for the NK cell/anti-CD16 system. These results demonstrate that immune receptor triggering can be influenced by the nanoscale spatial organization of receptor/ligand interactions.

Original publication

DOI

10.1021/nl403252x

Type

Journal article

Journal

Nano Lett

Publication Date

2013

Volume

13

Pages

5608 - 5614

Keywords

Antigens, CD3, Humans, Killer Cells, Natural, Nanoparticles, Nanotechnology, Receptor-CD3 Complex, Antigen, T-Cell, Receptors, Cell Surface, Receptors, IgG, Receptors, Natural Killer Cell, T-Lymphocytes