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Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system.

Original publication

DOI

10.1007/978-1-0716-2996-3_18

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2023

Volume

2632

Pages

259 - 267

Keywords

Long-read, Oxford Nanopore Sequencing, Single-cell, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Nanopores