Visium Cytassist accessible

The first image shows the Visium workflow, a spatial transcriptomics assay that measures gene expression across a tissue section while preserving its spatial organisation. The process begins with sample preparation and imaging. Next, the sample is processed using the CytAssist instrument. A magnified inset shows cells positioned over a grid, indicating how spatial information is preserved during this transfer. The workflow then proceeds to probe extension and library construction, where captured RNA is converted into DNA and prepared for sequencing. After sequencing, data are processing and visualisation completes the process.

The second image highlights the structure of a Visium Spatial Gene Expression Slide. A square capture area (approximately 6.5 mm by 6.5 mm) is surrounded by a Fiducial Frame of 8 mm by 8 mm which contains approximately 5,000 barcoded spots, each with a unique spatial barcode. A magnified view shows the arrangement of these barcoded spots.  A sequencing schematic of the Visium Gene Expression barcode spots highlights the components: gene sequence, Partial Read 1, spatial barcode, UMI, and a poly(dT) region.

The third image shows Visium HD Slide 6.5mm. A square capture area (approximately 6.5 mm by 6.5 mm) with continuous lawn of oligos is surrounded by a Fiducial Frame of 8 mm by 8 mm. A magnified view shows a grid of 2 x 2 micrometres barcoded squares binned to 8 x 8 micrometres.