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CosMx Spatial Molecular Imager (Bruker)

CosMx Spatial Molecular Imager (Bruker)

HOW DOES IT WORK?

CosMx SMI uses a cyclic single-molecule hybridization and imaging approach to detect RNA transcripts directly within intact tissue. The method begins with gene-specific probes that bind to target RNA, followed by fluorescently labelled secondary probes that amplify the signal for high sensitivity. After each imaging cycle, the fluorescent tags are removed, allowing the process to repeat multiple times. Across these cycles, each gene is assigned a unique combination of colour and positional “barcode,” enabling highly multiplexed detection of RNA molecules at subcellular resolution. This approach provides precise spatial localisation of transcripts and supports detailed mapping of cell states and tissue architecture.

 

CosMxSource: Bruker Spatial Biology 

Accessible version

APPLICATIONS
  • Targeted gene expression (with 1K and 6K RNA panels)
  • Whole Transcriptome gene expression (with WTX assay)
  • Multiomic gene and protein expression

COMPATIBLE SPECIES
  • Human
  • Mouse

SAMPLE COMPATIBILITY
  • Fresh Frozen 
  • FFPE
  • Tissue microarray (TMA)
  • Organoids

ASSAY INPUT
  • Tissues sectioned onto standard histology Superfrost Plus glass slides

INSTRUMENT RUN TIME
  • 5-11 days, depending on:

- plex size

- number of slides per run

- number of FOVs (0.51mm x 0.51mm) selected for the run (with a maximum of 800-1000 ROIs per run)

ANALYTICAL REGION
  • 15mm × 20mm / (0.51mm × 0.51mm/FOV)

SUBCELLULAR RESOLUTION
  • <50nm XY plane

CELL SEGMENTATION

Nuclei; membrane; proteins morphology marker

DETECTABLE GENES
  • Ready-to-use panels for CosMx RNA assays:

CosMx Human Whole Transcriptome Panel (WTX)
CosMx Human 6K Discovery Panel
CosMx Universal Cell Characterisation 1K RNA Panel
CosMx Mouse Neuroscience 1K Panel
CosMx Human Immuno-Oncology Panel

Please refer to Bruker’s webpage for product range.

  • Ready-to-use panels for CosMx Spatial Proteomics assays: 

CosMx Mouse Neuroscience Protein Panel (up to 68 protein targets)
CosMx Human Immuno-Oncology Protein Panel (>64 protein targets)

Please refer to Bruker’s webpage for product range.

  • Custom panel options available => possibility to add on:

Custom probes for RNA (up to 50 for 1K-plex, up to 200 for 6K-plex and WTX)
Custom targets for protein (up to 8, but please provide your own unconjugated Ab)