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Whole-organ confocal

ZEISS LSM 880 Whole-organ confocal microscope

GENERAL SPECIFICATIONS:

ZEISS LSM 880 confocal system on Axio Examiner upright fixed stage microscope
xy, xz, xyz, xyzt, lambda, tiling scan modes

MICROSCOPE OBJECTIVES:

Plan-APO 10X/0.45 WD=2.0
Plan-APO 20X/0.8

ILLUMINATION:

Colibri 7 LED illumination for epifluorescent imaging
405-nm laser, Argon laser (458-, 488-, 514-), 561-, 594- and 633-nm lasers

DETECTORS AND AVAILABLE FILTERS:

2 x PMT NDD detectors
34 descanned spectral channels

SOFTWARE:

Zeiss Zen software
Mosaic and stitching imaging

ACKNOWLEDGMENT:

We request that publications that include data or analysis performed with the facility include the following acknowledgment- “We acknowledge the generous support of the Kennedy Trust for Rheumatology Research for the microscopy facilities used in this research”.