TIRF
Olympus TIRF Microscope
GENERAL SPECIFICATIONS
- Olympus TIRF IX83 Microscope based on IX83 motorized inverted microscope
- Compensation focusing system
- CellVivo incubator system for live-cell imaging and temperature control
- 4 diode lasers
- Freely selectable ROI for imaging, bleaching and photoactivation
- Imaging speed of up to 4-10 ms per frame (512 x 512 pixels)
- Camera splitter for simultaneous imaging in two channels
- DIC optics
- Individual control over depth penetration of evanescent laser illumination
MICROSCOPE OBJECTIVES
- UAPON 20x /NA 0.70
- UCPLFLN 20x/NA 0.70
- UAPON 150x/NA 1.45 Oil
ILLUMINATION
- Lamps for bright field, DIC and epifluorescent imaging
- Violet Diode Laser 405 nm
- Blue Diode Laser 488 nm
- Green Diode Laser 561 nm
- Red Diode Laser 640 nm
DETECTORS
- Evolve EMCCD 512x512 camera supplemented with Cairn camera splitter (IX3 RSD) for simultaneous imaging in two channels
- Hamamatsu Flash 2.8 camera
ENVIRONMENT CONTROL
- Dark incubator chamber with temperature, CO2 control and lighting from CellVivo
SOFTWARE
- Olympus cellSens Dimension
ACKNOWLEDGEMENT
The TIRF microscope was purchased with Wellcome Trust grant 100262/Z/12/Z. Please acknowledge this in publications using this microscope.