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The Oxford-ZEISS CoE and Wellcome Centre for Human Genetics were awarded a MRC Multiuser Equipment Grant to purchase a ZEISS ELYRA 7 Lattice SIM. Accessible to users from the Kennedy, IDRM and beyond.

GENERAL SPECIFICATIONS

Elyra7-lattice-SIM

  • Structured illumination microscope (SIM) allowing super-resolved images of live samples
  • CO2 and thermal environment control
  • RAPP FRAP module for photo-manipulation

 

 

 

 

 

MICROSCOPE OBJECTIVES

  • C-Apochromat 63x/1.20 W Corr UV-VIS-IR
  • PApo 40x/1.4 Oil DIC (UV) V-IR
  • Plan-Apochromat 63x/1.40 Oil DIC
  • alpha PApo 63x/1.46 Oil Corr
  • Plan-Neofluar 10x/0.30 WD=5.2

ILLUMINATION

  • Halogen lamp 
  • 488 nm, 100 mW
  • 405 nm, 50 mW
  • 561 nm, 500 mW
  • 642 nm, 500 mW

DETECTORS AND AVAILABLE FILTERS

  • FL Filter Set 77 GFP/mRFP/Alexa 633
  • Filter Set 96 EX BP 390/40, BS FT 420, EM BP 450/40
  • pco.edge sCMOS

SOFTWARE

  • ZEISS Zen Black acquisition software
  • Dedicated analysis workstation
  • ZEISS Zen Blue analysis software

ACKNOWLEDGMENT

We request that publications that include data or analysis performed with the facility include the following acknowledgment- “We acknowledge the generous support of the MRC Equip grant MC_PC_MR/X012069/1, Kennedy Trust for Rheumatology Research, Wellcome Centre of Human Genetics and Carl Zeiss GMBH for the microscopy facilities used in this research.”.

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