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T-cell apoptosis is central to the resolution of chronic inflammation. Inhibition of this process of programmed cell death contributes to disease persistence in conditions such as rheumatoid arthritis. An understanding of T-cell apoptosis and its regulation is clearly important for understanding the pathophysiology of inflammatory disease. This chapter describes a number of apoptosis assays that can be used to measure T-cell apoptosis in synovial fluid. The choice of assay depends, in part, on the phase of apoptosis under investigation and this review puts this into context by introducing these phases and their regulation.

Original publication

DOI

10.1007/978-1-59745-402-5_9

Type

Chapter

Publication Date

2007

Volume

136

Pages

117 - 138

Keywords

Animals, Apoptosis, Biological Assay, CD3 Complex, Caspase 3, DNA, DNA Fragmentation, Humans, In Situ Nick-End Labeling, Inflammation, Mitochondria, Phosphatidylserines, Receptors, Cell Surface, Synovial Fluid, T-Lymphocytes