Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

MicroRNAs (miRNAs) have emerged as key players in the degradation of target mRNAs. They have been associated with diverse biological processes, and recent studies have demonstrated that miRNAs play a role in inflammatory responses. The identification of miRNA and their corresponding messenger RNA (mRNA) targets can therefore be very helpful. In this chapter, we first overview the field of miRNAs and then show the fundamental techniques for the identification of miRNAs and confirmation of their role on target mRNAs.

Original publication

DOI

10.1007/978-1-4939-0404-4_7

Type

Journal

Methods Mol Biol

Publication Date

2014

Volume

1142

Pages

55 - 63

Keywords

Animals, Arthritis, Rheumatoid, Blotting, Western, Cell Culture Techniques, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Humans, MicroRNAs, RNA, Messenger, Real-Time Polymerase Chain Reaction, Transfection