Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.
Rydzik AM., Leung IKH., Kochan GT., Thalhammer A., Oppermann U., Claridge TDW., Schofield CJ.
Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.