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Recombinant avipoxvirus vectors are attractive candidates for use in vaccination strategies for infections such as human immunodeficiency virus type 1 (HIV-1), where induction of a CD8+ T cell response is thought to be an important component of protective immunity. Here, we report the expression of a multiepitope polypeptide (TAB9) composed of the central 15 amino acids of the V3 loop from six different isolates of HIV-1 in a fowlpox virus (FWPV) vector, and the use of this vector (FPTAB9LZ) to induce strong HIV-specific CD8+ T cell responses in mice. In animals immunized twice intravenously with FPTAB9LZ, almost 2% of the CD8+ T cells in the spleen were shown to produce IFN-gamma in response to stimulation with HIV-1 peptides 1 week after the second immunization. The most dominant response was to the HIV-1 IIIB peptide. A strong HIV-specific response was also induced by intraperitoneal immunization of mice with FPTAB9LZ, whilst subcutaneous immunization elicited a weaker response. Intraperitoneal immunization with FPTAB9LZ was also shown to provide protection against challenge with a recombinant vaccinia virus expressing antigens, including those in TAB9. These results confirm the potential of FWPV vectors for use in HIV vaccination strategies.

Original publication

DOI

10.1089/08828240260066260

Type

Journal article

Journal

Viral Immunol

Publication Date

2002

Volume

15

Pages

337 - 356

Keywords

Animals, CD8-Positive T-Lymphocytes, Cell Line, Drug Administration Routes, Epitopes, Epitopes, T-Lymphocyte, Fowlpox virus, Gene Expression, Genetic Vectors, HIV Antigens, HIV Envelope Protein gp120, HIV-1, Humans, Injections, Intravenous, Mice, Peptide Fragments, Peptides, Recombination, Genetic, Spleen, T-Lymphocytes, Cytotoxic, Vaccination, Vaccinia virus