dipogenic Differentiation Assay for the Study of Insulin Signaling.
Gamwell JM., Cresswell E., Pinnick KE.
In vitro models of adipogenesis are valuable tools for the study of human adipocyte development and function. Prevailing methods rely on the extended use of supraphysiological concentrations of glucose and adipogenic hormones, which can negatively impact some aspects of adipocyte function, in particular, insulin signaling. In this chapter, we present a modified adipogenic differentiation protocol which has been optimized for the study of insulin signaling and glucose uptake in human adipose-derived stem cells (hADSCs).