Aria III Configuration
Overview
The Aria III is a digital system with fixed optical alignment. The KIR and ORC instruments are equipped with a Blue 488 nm, Red 633 nm and Violet 405 nm laser, capable of measuring up to 11 parameters in addition to FSCS and SSC. The KIR Aria III system has an additional Yellow-Green 561 nm laser that increases detection sensitivity for the PE fluorochrome, eliminates PE-FITC spillover and improves resolution of red fluorescence protein.
Detailed information
The Aria III has four nozzle sizes: 70 µm, 85 µm, 100 µm and 130 µm and is capable of sorting cells ranging from 5 to 40 µm in size at a flow rate of 5000 to 20000 cells per second.
The sorter offers four/two way cell sorting in a variety of tubes (0.2 ml, 0.5 ml, 5 ml and 15 ml) and single cell sorting in a variety of tissue culture plates using the Automated Cell Deposition (ACDU) unit.
You have the option to adjust the temperature to your requirement for both the sample and collection tubes. The sample agitation option helps cells from being pelleted to the bottom of the sample tube and hence improves the sort.
Facility staff run CS&T beads on a regular basis to monitor the performance of the instrument and to ensure the correct laser set up is in place.
Aria III 4 Laser System
LASER | COLOUR | FILTER | MIRROR |
---|---|---|---|
Blue 488 nm | FITC, Alexa 488 | 530/30 | 502 LP |
PerCP, PerCP-CY5.5 | 695/41 | 655LP | |
Yellow-Green 561 nm | PE | 582/15 | |
PE-Cy5 | 670/14 | 630LP | |
PE-Texas Red | 610/20 | 600LP | |
PE-Cy7 | 780/60 | 735LP | |
Red 633 nm | APC, Alexa 647 | 660/20 | |
APC-Cy7, APC-H7 | 780/60 | 755LP | |
APC-Alexa 700 | 730/45 | 690LP | |
Violet 405 nm | Pacific Blue, BV421 | 450/40 | |
AmCyan, BV510 | 510/50 | 502LP | |
BV605 | 610/20 | 595LP |
LASER | COLOUR | FILTER | MIRROR |
---|---|---|---|
Blue 488 nm | FITC, Alexa 488 | 530/30 | 502 LP |
PE | 585/42 | 556LP | |
PerCP, PerCP-CY5.5 | 695/40 | 665LP | |
PE-Texas Red | 616/23 | 610LP | |
PE-Cy7 | 780/60 | 735LP | |
Red 633 nm | APC, Alexa 647 | 660/20 | |
APC-Cy7, APC-H7 | 780/60 | 755LP | |
APC-Alexa 700 | 730/45 | 690LP | |
Violet 405 nm | Pacific Blue, BV421 | 450/40 | |
AmCyan, BV510 | 510/50 | 502LP | |
BV605 | 610/20 | 600LP |