Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes.
Herberg JA., Kaforou M., Gormley S., Sumner ER., Patel S., Jones KDJ., Paulus S., Fink C., Martinon-Torres F., Montana G., Wright VJ., Levin M.
We compared the blood RNA transcriptome of children hospitalized with influenza A H1N1/09, respiratory syncytial virus (RSV) or bacterial infection, and healthy controls. Compared to controls, H1N1/09 patients showed increased expression of inflammatory pathway genes and reduced expression of adaptive immune pathway genes. This was validated on an independent cohort. The most significant function distinguishing H1N1/09 patients from controls was protein synthesis, with reduced gene expression. Reduced expression of protein synthesis genes also characterized the H1N1/09 expression profile compared to children with RSV and bacterial infection, suggesting that this is a key component of the pathophysiological response in children hospitalized with H1N1/09 infection.