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Schistosoma mansoni, the parasite responsible for schistosomiasis, lacks the "de novo" purine biosynthetic pathway and depends entirely on the purine salvage pathway for the supply of purines. Numerous reports of praziquantel resistance have been described, as well as stimulated efforts to develop new drugs against schistosomiasis. Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a key enzyme of the purine salvage pathway. Here, we describe a crystallographic structure of the S. mansoni HPGRT-1 (SmHGPRT), complexed with IMP at a resolution of 2.8 Ǻ. Four substitutions were identified in the region of the active site between SmHGPRT-1 and human HGPRT. We also present data from RNA-Seq and WISH, suggesting that some isoforms of HGPRT might be involved in the process related to sexual maturation and reproduction in worms; furthermore, its enzymatic assays show that the isoform SmHGPRT-3 does not present the same catalytic efficiency as other isoforms. Finally, although other studies have previously suggested this enzyme as a potential antischistosomal chemotherapy target, the kinetics parameters reveal the impossibility to use SmHGPRT as an efficient chemotherapeutic target.

Original publication

DOI

10.1016/j.molbiopara.2019.02.005

Type

Journal article

Journal

Mol Biochem Parasitol

Publication Date

04/2019

Volume

229

Pages

24 - 34

Keywords

HGPRT, Hypoxanthine-guanine phosphoribosyltransferase, Purine salvage pathway, Schistosoma mansoni, Amino Acid Sequence, Animals, Catalytic Domain, Helminth Proteins, Hypoxanthine Phosphoribosyltransferase, Isoenzymes, Kinetics, Molecular Sequence Data, Reproduction, Schistosoma mansoni, Sequence Alignment