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Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung.

Original publication

DOI

10.1002/0471142956.cy1228s60

Type

Journal article

Journal

Curr Protoc Cytom

Publication Date

04/2012

Volume

Chapter 12

Keywords

Animals, Cell Movement, Diagnostic Imaging, Fluorescent Antibody Technique, Green Fluorescent Proteins, Instillation, Drug, Lung, Mice, Microscopy, Staining and Labeling