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Confocal

Olympus FV1200 Laser Scanning Microscope

 Olympus FV1200 Laser Scanning Microscope

GENERAL SPECIFICATIONS

  • FluoView Spectral  FV1200 Laser Scanning Microscope based on IX83 motorized inverted microscope
  • Compensation focusing system
  • CellVivo incubator system for live-cell imaging and temperature control
  • 4 diode lasers
  • Freely selectable ROI for laser scanning, bleaching and photoactivation
  • Scan speeds of up to 4 frames/second with 512 x 512 pixels
  • xy, xz, xyz, xyzt, lambda, line scan and spot scan modes
  • DIC optics
  • Diffusion Measurement Package included for FCS, FCCS, RICS and FRAP

MICROSCOPE OBJECTIVES

  • UPLSAPO 10x /NA 0.40
  • UPLSAPO 20x/NA 0.75
  • UPLSAPO 30x/NA 1.05 Silicone Immersion
  • UPLSAPO 60x/NA 1.4 Super Corrected
  • UPLSAPO 60x/NA 1.3 Silicone Immersion
  • UPLSAPO 60x/NA 1,2 Water Immersion 

ILLUMINATION

  • Lamps for bright field, DIC and epifluorescent imaging
  • Violet Diode Laser 405 nm (25 mW)
  • Blue Diode Laser 473 nm (50 mW)
  • Green DPSS Laser 559 nm (25 mW)
  • Red Diode Laser 635 nm (25 mW) 

DETECTORS

  • 3 PMT equipped with galvano grating spectral scanning systems for fluorescence emission detection with variable setting of wavelength band
  • 2 high sensitive GaAsP detection channels with filter sets 

ENVIRONMENT CONTROL

  • Dark incubator chamber with temperature, CO2 control and lighting from CellVivo

SOFTWARE

  • Olympus FluoView
  • Positioning (up to 3000 measuring points different in X, Y and Z)
  • Mosaic and stitching imaging
  • Time-lapse experiments
  • FCS, point FCCS, RICS and FRAP measurements

ACKNOWLEDGEMENT

We request that publications that include data or analysis performed with the facility include the following acknowledgement- “ We acknowledge the generous support of the Kennedy Trust for Rheumatology Research for the microscopy facilities used in this research”.