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Aria III Configuration

FACS Aria III

Overview

The Aria III is a digital system with fixed optical alignment. The KIR and ORC instruments are equipped with a Blue 488 nmRed 633 nm and Violet 405 nm laser, capable of measuring up to 11 parameters in addition to FSCS and SSC. The KIR Aria III system has an additional Yellow-Green 561 nm laser that increases detection sensitivity for the PE fluorochrome, eliminates PE-FITC spillover and improves resolution of red fluorescence protein.  

Detailed information

The Aria III  has four nozzle sizes: 70 µm, 85 µm, 100 µm and 130 µm and is capable of sorting cells ranging from 5 to 40 µm in size at a flow rate of 5000 to 20000 cells per second.

The sorter offers four/two way cell sorting in a variety of tubes (0.2 ml, 0.5 ml, 5 ml and 15 ml) and single cell sorting in a variety of tissue culture plates using the Automated Cell Deposition (ACDU) unit.  

You have the option to adjust the temperature to your requirement for both the sample and collection tubes.  The sample agitation option helps cells from being pelleted to the bottom of the sample tube and hence improves the sort.

Facility staff run CS&T beads on a regular basis to monitor the performance of the instrument and to ensure the correct laser set up is in place.  

FACS Aria III sorts

 

 Aria III 4 Laser System

LASER COLOUR  FILTER MIRROR
Blue 488 nm FITC, Alexa 488 530/30 502 LP
  PerCP, PerCP-CY5.5 695/41 655LP
Yellow-Green 561 nm PE 582/15  
  PE-Cy5 670/14 630LP
  PE-Texas Red 610/20 600LP
  PE-Cy7 780/60 735LP
Red 633 nm APC, Alexa 647 660/20  
  APC-Cy7, APC-H7 780/60 755LP
  APC-Alexa 700 730/45 690LP
Violet 405 nm Pacific Blue, BV421 450/40  
  AmCyan, BV510 510/50 502LP
  BV605 610/20 595LP

 

Aria III 3 Laser System
LASER COLOUR FILTER MIRROR
Blue 488 nm FITC, Alexa 488 530/30 502 LP
  PE 585/42 556LP
  PerCP, PerCP-CY5.5 695/40 665LP
  PE-Texas Red 616/23 610LP
  PE-Cy7 780/60 735LP
Red 633 nm APC, Alexa 647 660/20  
  APC-Cy7, APC-H7 780/60 755LP
  APC-Alexa 700 730/45 690LP
Violet 405 nm Pacific Blue, BV421 450/40  
  AmCyan, BV510 510/50 502LP
  BV605 610/20 600LP