Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Characterisation of tyrosine phosphorylations induced in immune cells in response to inflammatory stimuli may help elucidate the molecular bases of the diversity of immune responses. We have used anti-phosphotyrosine antibodies in combination with cell surface biotinylation in a two-step affinity purification procedure to recover pervanadate-induced tyrosine phosphorylated proteins from sub-cellular compartments, including the cell surface, of murine T cells and macrophages prior to separation by solution-phase isoelectric focussing and one-dimensional gel electrophoresis and identification by tandem mass spectrometry.

Original publication

DOI

10.1002/pmic.200401176

Type

Journal article

Journal

Proteomics

Publication Date

06/2005

Volume

5

Pages

2417 - 2421

Keywords

Animals, Antibodies, Biotinylation, Cell Compartmentation, Cell Line, Electrophoresis, Polyacrylamide Gel, Hybridomas, Immunoprecipitation, Isoelectric Focusing, Macrophages, Mass Spectrometry, Membrane Proteins, Mice, Phosphoproteins, Phosphorylation, Phosphotyrosine, Protein Tyrosine Phosphatases, Proteome, T-Lymphocytes, Vanadates