Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Immune cells rely on the generation of mechanical force to carry out their function. Consequently, there is a pressing need for quantitative methodologies that permit the probing of the spatio-temporal distribution of mechanical forces generated by immune cells. In this chapter, we provide a guide to quantify immune cell force generation using traction force microscopy (TFM), with a specific focus on its application to the study of the T-cell immunological synapse.

Original publication

DOI

10.1007/978-1-0716-3135-5_23

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2023

Volume

2654

Pages

363 - 373

Keywords

Biophysics, Cytoskeleton, Immunological synapse, Mechanical forces, Mechanobiology, T cell activation, Traction force microscopy, Microscopy, Atomic Force, Traction, Mechanical Phenomena, Immunological Synapses